While conducting my experiment, I completed ten trials for each type of detergent.The additional trials were completed to reduce error in my results.I utilized a graduated test tube to measure the amount of visible extracted DNA.The measurements confirmed my hypothesis that Ajax dishwashing liquid extracted the most visible DNA.
Difficulty of the Project
Time Taken to Complete the Project
The purpose of the experiment is to test the effectiveness of a dishwashing liquid, a laundry detergent, and a shampoo during a DNA extraction process from a strawberry.
- Three 250 ml tri-pour beakers
- One glass extraction rod
- One plastic 4 oz squeezing bottle
- 10 graduated test tubes
- 91% isopropyl alcohol
- Blue monster DNA stain
- Powder buffer (which contains)-
- DNA suspension agent
- PH stabilizer
- Protein destroyer
- Nylon fast flow filter
- Measuring spoons
- 4 pipettes
- Purex laundry detergent
- Fructis shampoo
- Ajax dishwashing liquid
- Test Tube Holder
- 3 Bowls
- Distilled water
Deoxyribonucleic acid, also abbreviated as DNA, is the substance in the cells of living things that contains the information of life (Wilcox, 8).Among animals, plants, and microorganisms, there are many kinds of cells that have different shapes and perform different functions (Ruiz, 12).Plant and animal cells are eukaryotic cells, which mean that the nucleus is isolated from the cytoplasm by a membrane called the nuclear membrane (20).The DNA is located inside the nucleus.
When a person is eating a strawberry, they do not realise they are eating strawberry DNA!A strawberry is an octoploidWhich means has eight sets of seven chromosomes makes its genetics very complex. Scientists are therefore working on wild diploid strawberries which are easier to analyze, and then seek to apply knowledge across to the cultivated strawberry.
Buffer – A solution that protects or “buffers” a reaction against changes in pH.
DNA - Deoxyribonucleic Acid, the substance of inheritance, which directs the growth, organization, development, and function of cells.
I predict that the use of Ajax dishwashing liquid will be the best at breaking down the cell membrane and allow the greatest amount of DNA to become visible.I believe this because I have observed the way dishwashing liquid breaks down the grease on pots and pans while cleaning the dishes.
To perform my experiment I completed the following steps:
- Add 4 oz of distilled water to the beaker
- Add 1 ¼ oz (6.25 ml) of powdered buffer into the beaker
- Stir until buffer is dissolved in distilled water
- Add 5 ml of detergent to mixture, and stir for 2- 4 minutes, slowly (Try not to create suds)
- Put mixture into ice and chill
- Mash 5 strawberries in a blender
- Warm up a cup of hot water at 60 degrees Celsius
- Put “mush bag” into water
- Set it in hot water for 10 minutes
- Put 5 tbsp (75 ml) of mush into a beaker
- Put 10 tbsp of chilled buffer into beaker
- Stir mush with chilled buffer for 4 minutes
- Pour the mush into the beaker with the filter
- Add 5 ml of “liquid from mush” into each of ten test tubes
- Add 5 more ml of alcohol to test tubes
- Put 1 drop of Blue DNA Stain into each beaker
- Observe white, slimy DNA
- Measure your results
During my experiment the following made up my controls:
- Distilled water
- Powdered Buffer
The following was my variable:
- Type of detergent
I performed the following three sets of experiments 10 times:
- DNA extraction with Ajax dishwashing liquid
- DNA extraction with Purex laundry detergent
- DNA extraction with Fructis shampoo
The ten Ajax experiment trials resulted in measured extractable DNA from 0.250 to 1.500 milliliters with an average extracted DNA of 0.775 milliliters.The use of Ajax produced the most consistent results.Six of the ten trials produced 0.750 milliliters of visible DNA.
The ten Purex experiment trials resulted in measured extractable DNA from 0.250 to 1.750 milliliters with an average extracted DNA of 0.625 milliliters.Out of all 30 trials for all three detergents, one of the Purex trials had the highest amount of visible DNA, which was 1.750 milliliters.Even though Purex produced one of the largest quantities of visible DNA in one trial, the remaining nine trials did not produce consistent results.
The ten Fructis experiment trials resulted in measured extractable DNA from 0.100 ml to 1.500 milliliters with an average extracted DNA of 0.485 milliliters.Two of the Fructis trials had the lowest amount of visible DNA, which was 0.100 milliliters.The Fructis trials produced the same inconsistencies similar to the Purex trials.
Avg DNA ml
At the conclusion of the experimental trials, I found that my hypothesis was correct.I had predicted that Ajax dishwashing liquid would produce the most extractable DNA.The measured results showed that Ajax produced almost two times more DNA than Fructis Shampoo and had the most consistent results for each trial. The thirty separate trials went smoothly and I had no problems
.An interesting future experiment might involve changing the stage of ripeness, using different temperatures, or levels of sun exposure.Another study could involve the use of different types of fruits and vegetables.This experiment taught me that every human and plant cell contains DNA.
“DNA Is Like A Library Of Instructions”
2. http://www.ehow.com/how-does_5149970_dna-extracted-examined.html “How Is DNA Extracted and Examined?”
“Methods of DNA Extraction in Plants”
Ma Wen Jie
Eukaryotes= Animal and Plant Cells
Nigel D. Purchon
“Strawberry Processing Techniques:Freezing and Freeze-Drying”
Dr. Harmit Singh
“Strawberry Plant Scientific Classification”
7.Illustrated Science Encyclopedia
“DNA” and “Detergent”
8. DNA The Thread of Life
Frank H. Wilcox
Lerner Publications Company
Lynette Brent Sandvold
Lucent Books, Inc.
11. Life Lines: The Story of New Genetics
J. S. Kidd and Renee A. Kidd
Facts On File, Inc.
12. The Life of a Cell
Andres Llamas Ruiz
Sterling Publishing Co., Inc.
13. DNA: The Ladder of Life
McGraw-Hill Book Company